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Environmental DNA (eDNA) Methods

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Environmental DNA (eDNA) Methods

November 27 @ 9:00 am - 5:00 pm PST

PLEASE NOTE that due to low registration numbers this course is cancelled and registered participants will be offered the opportunity to move into a course at an alternative location. Please contact Darren Hebert of the Natural Resource Training Group should you have questions pertaining to this course.
Date: November 27, 2018
Time: 9am-5pm

Location: Whistler, BC
Venue: TBD
Course Fee: $425.00
Pre-requisites: There are no formal prerequisites.
Personal Equipment Requirements:  There are no personal equipment requirements for course participants.

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Description

Environmental DNA (eDNA) is a relatively new method that is increasingly being used for survey of aquatic taxa, including vertebrates, invertebrates, and plants. This highly effective, cost-efficient and non-invasive method relies on the detection of genetic materials in aquatic ecosystems using laboratory analysis of water samples to detect DNA from the target taxa.

Environmental DNA methods are predicated on basic biological principles, and employ long-standing proven methods for laboratory testing. Quantitative polymerase chain reaction (qPCR) is the preferred method for detecting DNA in water samples as it provides an effective way to amplify, detect and identify existing DNA in sample water collected from natural aquatic systems.  

This one-day course delivered by the Natural Resources Training Group will provide training and instruction for participants’ eager to engage in the use of eDNA methods. The training will benefit those interested in conducting or contracting eDNA field programs. Managers responsible for over-seeing contracts for eDNA projects will also benefit by attending this course.

In the first part of the course we’ll provide background on the principles, and theory, inherent to standard eDNA applications. The second part of the course provides formal training on field and lab protocols; including data interpretation and reporting standards.  The course will inform participants to support effective survey design, implementation and reporting. We’ll also employ a ‘hands-on’ approach to tutor participants and ensure competency in field collection treatment (filtering and dispatching samples for analysis).

The training includes updates on new (January 2018) protocols (Hobbs et al 2018), and lab methods described in a recent publication by University of Victoria and Jared Hobbs (Veldhoen et al 2016). Course instruction will also help proponents evaluate eDNA proposals to ensure quality during implementation and lab selection. Attendance is strongly encouraged for staff who might find themselves conducting or supervising eDNA projects.

Objectives

By the end of this course, course participants will be able to:

  • Identify limitations and advantages of eDNA, including its use relative to conventional survey methods
  • Recognize opportunities where eDNA methods may confer efficiencies, in time and cost, relative to conventional methods.
  • Design and conduct an eDNA study and be familiar with field sampling methods and protocol to ensure successful project delivery.
  • Identify procedural requirements for safe handling, transport and storage of eDNA samples.

Identify and meet project requirements, from ‘cradle-to-grave’ including considerations for primer development, sample design, project budgeting, implementation and reporting.

Course format

This one-day classroom-based course will include both lecture, demonstration and practical ‘hands on’ training in the standards and applications for lab and field eDNA procedures.

In the first part of the course we’ll provide background on the principles, and theory, inherent to standard eDNA applications. The second part of the course provides formal training on field and lab protocols; including data interpretation and reporting standards.  The course will inform participants to support effective survey design, implementation and reporting. We’ll also employ a ‘hands-on’ approach to tutor participants and ensure competency in field collection treatment (filtering and dispatching samples for analysis).

Potential Students

This training will benefit those interested in conducting or contracting eDNA field programs. Managers responsible for over-seeing contracts for eDNA projects will also benefit by attending this course.

Venue

Whistler
Whistler, BC V0N1B0 Canada + Google Map

Organizer

Natural Resources Training Group
Phone:
(250) 739-9113
Email:
info@nrtraininggroup.com
Website:
http://www.nrtraininggroup.com